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Eurofins DiscoverX Insulin Receptor Protein, Activated
Activated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.
Brand: Eurofins DiscoverX 14-803
Additional Details : Weight : 4.70710kg
Description
The insulin receptor kinase (IRK) is composed of two extracellular a-subunits (135 kDa), which contain the insulin-binding site, and two intracellular b-subunits (95 kDa), containing the protein tyrosine kinase domain. After insulin binding, the insulin-IRK complex internalizes into muscle and fat cells. The IRK substrates of IRS-1 and IRS-2 form complexes, through Src homology region 2 (SH2) domains, with docking molecules such as phosphoinositde-3-kinase (PI3K). The recruitment of PI3K in turn activates phosphoinositide-dependent kinases, which serve in the activation of protein kinase B (Akt) and atypical isoforms of protein kinase C (PKC). These activated kinases then phosphorylate downstream effectors, ultimately promoting the translocation of insulin-sensitive glucose transporter subtype 4 (GLUT-4) to the plasma membrane, to operate in the coordination of glucose metabolism.
GenBank NM_000208
Specifications
| mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol, 5 mM β-glycerophosphate, 1 mM Na3VO4. Frozen solution. | |
| 10 μg | |
| Recombinant | |
| Recombinant enzyme expressed in Sf21 insect cells | |
| ≥79% |
| 36.8 kDa | |
| Human | |
| N-terminal 6His | |
| Truncated | |
| Insulin Receptor, activated |