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Invitrogen™ IL-6R/IL-6RA Mouse ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EMIL6RAX5
Additional Details : Weight : 0.33800kg
Description
The Mouse Interleukin-6 RA (Ms IL-6R/IL-6RA) ELISA quantitates Ms IL-6RA in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-6RA. Principle of the method The Mouse IL-6RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL6R (interleukin-6 receptor subunit alpha) is a part of the receptor for interleukin 6. The IL-6 receptor complex is composed of two membrane glycoproteins: the low affinity receptor and the signaltransducing component. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.Specifications
P22272 | |
6 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
5 x 96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
16194 | |
<12% | |
Standard, Wash Buffer, Chromogen, Biotinylated Detection Antibody, Assay Diluent Concentrate, Pre-coated 96 well plates, SAV-HRP, Adhesive Plate Covers, Stop Solution | |
5 x 96 Tests | |
Immunology | |
2°C to 8°C | |
Mouse | |
4 hr. 45 min. |
6.14 pg/mL to 1500 pg/mL | |
6 pg/mL to 1500 pg/mL | |
Biotin | |
Cytokines & Receptors | |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF | |
Mouse | |
Colorimetric Microplate Reader | |
CD126,IL-6 R,IL-6 R-alpha,IL-6 RA,Il6r | |
<10% | |
HRP | |
RUO | |
Plasma, 5 μ;L; Serum, 5 μ;L; Supernatant, 100 μ;L | |
CD126, IL-6 R, Il6r | |
1 hr. 20 min. |