IL-1 beta, clone: CRM56, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7018-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The CRM56 antibody reacts with human and baboon interleukin-1 beta; (IL-1 beta). IL-1 beta is a 17kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.Applications Reported: The CRM56 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry. Functional Grade purified CRM56 (cat. 16-7018) is recommended for use in functional assays.Applications Tested: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1 beta (IL-1 beta) in combination with the biotin CRM57 (13-7016) antibody for detection and recombinant human IL-1 beta (14-8018) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1 beta bioactivity.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Interleukin-1 beta, Catabolin, IL1B|
|ELISA, Flow Cytometry, Functional Assay, Neutralization|
|Store at 2-8°C.|
For Research Use Only.