Rat IgG2b kappa, PE-Cyanine7, clone: eB149/10H5, Isotype Control, eBioscience™
Rat Isotype Control
Brand: Affymetrix eBioscience 25-4031-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: PE-Cy7 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG2b K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes. It should be used at the same concentration as the experimental antibody.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Rat IgG2b kappa|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IgG2, IgG2b, IgG2bk|
|IgG2b, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!, handling and experimental procedures.|
|Control, Flow Cytometry|
For Research Use Only.