IFN gamma, Alexa Fluor 488, clone: XMG1.2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 53-7311-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Alexa Fluor 488|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.