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Molecular Probes™ HCS LIVE/DEAD™ Green Kit
HCS LIVE/DEAD™ Green Kit
Brand: Molecular Probes™ H10290
528.02 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Additional Details : Weight : 0.00450kg
Description
The HCS LIVE⁄DEAD™ Green Kit measures cytotoxicity by high content analysis with the Image-iT™ DEAD Green™ viability stain – a non-fluorescent and cell impermeant compound that exhibits a strong fluorescent enhancement upon binding to DNA. Drugs and test compounds leading to serious cell injuries, including plasma membrane permeability, allow entry of the Image-iT™ DEAD Green™ viability stain. This two-color fluorescence assay also employs the use of a cell permeant nuclear segmentation tool that stains both live and dead cells, reflecting the total cell number within the sample. For total cell staining, the kit includes both a blue-fluorescent Hoechst 33342 and a near infrared-fluorescent, HCS NuclearMask™ Deep Red reagent. Either of these segmentation tools may be used with Image-iT™ DEAD Green™ viability stain for excellent discrimination of live and dead cells that survives fixation and permeabilization for combination with additional parameters of cytotoxicity or other biomarkers. The kit contains sufficient reagents for performing 2, 96-well plates.Specifications
Blue, Far-Red, Green | |
HCS LIVE/DEAD™ Green Kit, Blue/Far-Red/Green, 96-Well Plate Format, Fluorescent Detection Method, 350/461, 488/515, 638/686nm Excitation/Emission, Used For Cell Viability, Proliferation & Function, Cellular Imaging | |
Cell Analysis, Cellular Imaging, High-content Screening (HCS), Cell Viability, Proliferation and Function, Cell Viability and Cytotoxicity | |
HCS NuclearMask™ deep red stain, Hoechst 33342, Image-iT™ DEAD green viability stain, classic dyes, other label(s) or dye(s) | |
96-well plate | |
1 Kit(s) |
≤–20°C | |
Fluorescent | |
Fluorescence Microscope, High Content Analysis Instrument | |
350, 488, 638nm | |
Blue-fluorescent hoechst 33342 and a near infrared-fluorescent, HCS NuclearMask™ deep red reagent |
For Research Use Only. Not for use in diagnostic procedures.