Invitrogen™ Glucose Colorimetric Detection Kit

The Glucose Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of glucose in serum, plasma, urine, buffers and tissue culture media.

This complete, ready-to-use kit includes clear 96-well plate(s), glucose standard, assay buffer, and other components to perform the assay. A 96-well microplate reader capable of reading optical density at 560 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, urine, buffers, and tissue culture media
• Sensitivity: 0.413 mg/dL
• Standard curve range: 0.5–32 mg/dL
• Reactivity: species independent

Background
Glucose is by far the most common carbohydrate. It is a monosaccharide, an aldose, a hexose, and a reducing sugar and is also known as dextrose, because it is dextrorotatory (rotates polarized light clockwise). The structure of glucose is shown below as both the straight chain and cyclic forms. For all biological and molecular events and for multiple cellular functions, energy is essential.

Energy is available in the form of ATP (adenosine triphosphate), most of which is generated through aerobic cellular respiration of carbohydrate and glucose, the major source of biological free energy in higher organisms. Reduced energy levels threaten cellular homeostasis and integrity. Impaired energy metabolism may trigger pro-apoptotic signaling (programmed cell death), oxidative damage, excitotoxicity, and impede mitochondrial DNA repair.

Assay principle
The Glucose Colorimetric Detection Kit is designed to quantitatively measure glucose in a variety of samples. A beta-D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the colorimetric substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room temperature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorimetric substrate to convert the colorless substrate into a pink-colored product. The pink product is read at 560 nm. Increasing levels of glucose cause a linear increase in color.