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Cytiva RNAspin 96 RNA Isolation Kit

For fast and efficient purification in a 96-well format

Brand:  Cytiva 25-0500-75

Additional Details : Weight : 2.32000kg Transport : UN number : 1993 Chem class : 3 Pack group : III


Product Code. 11763299

  • 1476.00 EUR / Pack of 4
Estimated Shipment: 15-04-2024
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Includes: Protocol and sufficient quantities of the following to carry out the number of preps indicated: DNase 1, RNase-free water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and buffers

Description

Description

  • 96 samples parallel-processed via either centrifugation or vacuum with high reproducibility in under 70 minutes
  • Can be used to isolate total RNA from cultured cells, tissue, bacteria or yeast
  • DNase I treatment removes genomic DNA resulting in high-quality total RNA product
  • Isolated RNA is of sufficient quality (A260/A280 = 1.8-2.2) and quantity (up to 100μg) for multiple downstream experiments: quantitative reverse transcription polymerase chain reaction (QRT-PCR), primer extension and RNase protection assays
  • Integrated wash plate eliminates risk of contamination
  • Accessory tissue plate allows tissue samples to be processed
  • Sample size: up to 30mg for animal tissue, up to 2 x 106 cells for cell culture (vaccum); 1x 107 cells for cell culture (centrifuge); Elution volume: 50μ-130μL

Specifications

Specifications

illustra RNAspin 96 Kit
Used to reproducibly (well-to-well, plate-to-plate) isolate total RNA from cultured cells, tissue, bacteria or yeastCan also be used in a fully automated flow with vacuum on common laboratory workstationsSuitable for direct use in downstream application
4 x 96 Preps
1.8-2.2
70 min/4 preps
96-well Plate
Protocol and sufficient quantities of the following to carry out the number of preps indicated, DNase 1, RNase-free Water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and Buffers
illustra RNAspin 96 Kit
10 mg tissue-30 mg tissue
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Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H312-Harmful in contact with skin.
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : deoxyribose nuclease, thiocyanic acid, ethanol
  • UNKNOWN ACUTE-27.5 per cent of the mixture consists of component(s) of unknown acute toxicity.

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