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Cytiva (Formerly GE Healthcare Life Sciences) HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Brand:  Cytiva (Formerly GE Healthcare Life Sciences) 17-5248-02

Additional Details : CAS Number : 64-17-5 Weight : 0.14400kg

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Product Code. 11773209

  • 662.00 EUR / 25mL

In stock, ready to ship: 3
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  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.



HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
3 to 12
5 bar [0.5 MPa] (70 psi)
4° to 30°C
4 mL/min (1 mL), 5 mL/min (5ml)
5 x 5 mL
HisTrap HP, 5 x 5 mL
5 x 5mL
6% Highly Cross-linked Agarose
Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .

Supplemental information
  • MIXTURE LIST-Contains : Ethanol, Nickel