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Gibco™ FreeStyle™ 293 Expression Medium

FreeStyle™ 293 Expression Medium

Brand:  Gibco™ 12338002

Additional Details : Weight : 21.59000kg

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Product Code. 15285885

  • 2015.00 EUR / 20 L


In Stock 17
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Description

Description

FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco™ FreeStyle™ 293 Expression Medium features:

  • Complete and ready-to-use format containing GlutaMAX™ supplement
  • Ability to support suspension growth and transfection of 293-F cells
  • Animal origin–free, protein-free and chemically defined formulation
  • Easy use with the FreeStyle™ 293 Expression systems
  • Scalability in spinner flasks and bioreactors

    Complete and ready-to-use format containing GlutaMAX™ supplement
    Gibco™ FreeStyle™ 293 Expression Medium is ready to use. It does not require the addition of serum, glutamine, or surfactants. Gibco™ FreeStyle™ 293 Expression Medium contains GlutaMAX™ supplement to minimize toxic ammonia build-up and improve cell viability and growth in an easy-to-use format.

    Ability to support suspension growth and transfection of 293-F cells
    Gibco™ 293-F, 293-H, and FreeStyle™ 293-F cells in suspension can be cultured directly into Gibco™ FreeStyle™ 293 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ FreeStyle™ 293 Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco™ FreeStyle™ 293 Expression Medium can be transfected using lipid based transfection reagents, such as 293fectin™ reagent and Freestyle™ MAX reagent.

    Animal origin–free, protein-free and chemically defined formulation
    Gibco™ FreeStyle™ 293 Expression Medium is animal origin–free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

    Easy use as part of the FreeStyle™ 293 Expression System and FreeStyle™ MAX Expression System for protein production
    The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein (see figure). The FreeStyle™ 293 Expression System combines Gibco™ FreeStyle™ 293 Expression Medium with 293fectin™ reagent, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. The FreeStyle™ MAX Expression System is a breakthrough technology for rapid and high-yield mammalian protein production. The FreeStyle™ MAX Expression System combines Gibco™ FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells.

    Scalability in spinner flasks and bioreactors
    Protein production using Gibco™ FreeStyle™ 293 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco™ FreeStyle™ 293 Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture.

    Universal bag packaging
    Gibco™ universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization.

    Product use
    Customers using Gibco™ FreeStyle™ 293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

    cGMP manufacturing and quality system
    Gibco™ FreeStyle™ 293 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
  • Specifications

    Specifications

    Sterile
    ISO 13485 standards, FDA
    FreeStyle™ 293 Expression Medium, 20L, 1X Concentration, Liquid, 7 to 7.6 pH, FreeStyle™ 293-F Cells Cell Type, 12 Months Shelf Life, Ambient Shipping Condition, 2 to 8°C Storage Requirements, For Antibody and Cell Culture Production, Mamm
    Antibody and cell culture production, mammalian cell culture, protein expression, viral vaccine production, cell culture, protein biology, mammalian expression, mammalian protein production, bioproduction, viral vaccine production
    With additives GlutaMAX™-I, sodium pyruvate, sodium bicarbonate, without additives phenol red
    7 to 7.6
    Cell culture grade
    For research use only
    GlutaMAX™-i, sodium pyruvate, sodium bicarbonate
    2 to 8°C
    FreeStyle™ 293-F cells
    1X, 1:1 Ratio
    Low
    Liquid
    1 Each
    FreeStyle™ 293 Expression Medium
    20L
    2 to 8°C, room temperature
    Phenol red
    Videos
    Documents

    For Research Use Only. Not for use in diagnostic procedures.