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FOXP3, eFluor 660, clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5773-82

Additional Details : Weight : 0.25000kg

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Product Code. 15391920

  • 311.00 EUR / 100µg


In Stock 5
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Description

Description

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human. Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523). The use of other fixation and staining buffers is not recommended.Applications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This FJK-16s antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please refer to intracellular Staining Protocol on Best Protocol web page - Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This FJK-16s antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Foxp3
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q99JB6
AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Canine, Feline, Murine, Porcine, Rat
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Frozen)
FJK-16s
eFluor 660
Foxp3
Liquid
Foxp3
IgG2a, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
100037405, 20371, 317382, 444998, 491876, 506053
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