missing translation for 'onlineSavingsMsg'
Learn More

FOXP3, eFluor 570, clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-5773-82

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15391070

  • 244.00 EUR / 100µg


In Stock 1
Add to basket

Description

Description

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.Applications Reported: This FJK-16s antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This FJK-16s antibody has been tested by immunohistochemistry of frozen mouse spleen at less than or equal to 5 μg/mL. The FJK-16s antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using high or low pH antigen retrieval and can can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

Foxp3
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q99JB6
AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Canine, Feline, Murine, Porcine, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
FJK-16s
eFluor 570
Foxp3
Liquid
Foxp3
IgG2a, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
100037405, 20371, 317382, 444998, 491876, 506053
Videos

For Research Use Only.