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Thermo Scientific™ HphI (10 U/μL)

The HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer (Isoschizomers: AsuHPI).

65.50 EUR - 270.00 EUR

Specifications

Concentration 10 U/μL
Methylation Sensitivity Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme HphI
Compatible Buffer 10X Buffer B
Sensitive to Heat Inactivation Yes
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10679900
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Thermo Scientific™
ER1101
300 U
65.50 EUR
300 units
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10292720
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Thermo Scientific™
ER1102
1500 U
270.00 EUR
1500 units
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Description

Description

Lambda DNA (dam- digested with HphI, 1.4% agarose, 168 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality -stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities
Specifications

Specifications

10 U/μL
HphI
Yes
Yes
Scarless Cloning
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
10X Buffer B
37°C
Restriction Enzyme
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.