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Thermo Scientific™ FastDigest MunI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

35.73 EUR - 90.50 EUR

Specifications

Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme MunI
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat Inactivation No
Optimal Reaction Temperature 37°C
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10344680
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Thermo Scientific™
FD0754
50 μL (50 Reactions)
90.50 EUR
50 reactions
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Thermo Scientific™
FD0753
20 μL (20 Reactions)
35.73 EUR
20 reactions
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Description

Description

Lambda DNA digested with MfeI (MunI), 0.7% agarose, 8 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Thermo Scientific FastDigest MunI restriction enzyme recognizes C^AATTG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: MfeI.

Thermo Scientific FastDigest MunI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Specifications

Specifications

Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X FastDigest Buffer/FastDigest Green Buffer
37°C
Restriction Enzyme
FastDigest™
MunI
No
No
Traditional Cloning
5' protruding ends
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.