Cytokeratin 7, eFluor 615, clone: LP5K, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 42-9005-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: This LP5K monoclonal antibody reacts with human cytokeratin 7 (K7), a 54-kDa type II (or basic) keratin expressed either alone or paired with cytokeratin 19 in simple epithelia, mesothelium, urothelium, and pseudostratified epithelium. Expression of cytokeratin 7 in the gastric foveolar, intestinal, and stratified squamous epithelia is extremely low or undetectable. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. In addition, most carcinomas express cytokeratin 7. The coordinated expression of this keratin with cytokeratin 20 is commonly used as a diagnostic marker for a variety of carcinomas.Applications Reported: This LP5K antibody has been reported for use in immunohistochemical and immunocytochemical staining.Applications Tested: This LP5K antibody has been tested immunocytochemistry on fixed and permeabilized MCF7 cells at less than or equal to 10 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Keratin, type II cytoskeletal 7, CK-7, Keratin-7, K7, Sarcolectin|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.