CD8b, eFluor 450, clone: eBioH35-17.2 (H35-17.2), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 48-0083-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.Applications Reported: This H35-17.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H35-17.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IgG2b, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.