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CD8a Monoclonal Antibody (53-6.7), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

86.75 EUR - 453.00 EUR

Specifications

Antigen CD8a
Clone 53-6.7
Concentration 1 mg/mL
Applications Flow Cytometry, Functional Assay, Neutralization
Classification Monoclonal
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Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15248367
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Affymetrix eBioscience
16-0081-82
100 μg
131.00 EUR
100µg
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Estimated Shipment: 08-05-2024
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15258367
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Affymetrix eBioscience
16-0081-85
500 μg
251.00 EUR
500µg
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Estimated Shipment: 08-05-2024
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15268367
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Affymetrix eBioscience
16-0081-86
1 mg
453.00 EUR
1mg
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Estimated Shipment: 10-04-2024
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15238367
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Affymetrix eBioscience
16-0081-81
50 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis. It has also been reported in T cell activation, isolation and depletion of CD8+ cells, and blocking of ligand binding. Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.
Specifications

Specifications

CD8a
1 mg/mL
Monoclonal
Liquid
RUO
PBS with no preservative; pH 7.2
Primary
4° C
53-6.7
Flow Cytometry, Functional Assay, Neutralization
Functional Grade
Rat
Mouse
IgG2a κ
Affinity chromatography
Antibody
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