CD83, eFluor 660, clone: Michel-17 (Michel17), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-0831-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The Michel-17 monoclonal antibody reacts with mouse CD83, a 45kDa cell surface glycoprotein and a member of the Ig superfamily. The mouse CD83 antigen is expressed predominantly on mature DC and activated lymphocytes. Cross-linking of CD83 with Michel-17 on DC or activated T cells does not induce any activation signal. CD83 plays an important role in T cell development through interaction with its ligand. CD83-Ig protein has revealed the presence of a CD83 ligand expressed mainly by B220+ cells in mouse spleen.Applications Reported: This Michel-17 (Michel17) antibody has been reported for use in flow cytometric analysis.Applications Tested: This Michel-17 (Michel17) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.