CD54 (ICAM-1) Rat anti-Mouse, Functional Grade, Clone: eBioKAT-1 (KAT-1, KAT1), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-0542-81
Additional Details : Weight : 0.01000kg
DescriptionA test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
|PBS with no preservative; pH 7.2|
|BB2, P3.58, ICAM-1, cell surface glycoprotein P3.58, intercellular adhesion molecule 1, human rhinovirus receptor, major group rhinovirus receptor|
|Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization|
|eBioKAT-1 (KAT-1, KAT1)|
For Research Use Only.