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CD45, Alexa Fluor 532, clone: 30-F11, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 58-0451-80

Additional Details : Weight : 0.25000kg

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Product Code. 15392160

  • 105.00 EUR / 25µg


Estimated Shipment date
from Supplier 27-07-2018
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Description

Description

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone mmarrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser
Specifications

Specifications

CD45
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06800
Cluster designation 45, common leukocyte antigen, T220 and B220
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
30-F11
Alexa Fluor 532
Ptprc
Conjugated
Ptprc
IgG2b, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
19264
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