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CD45 Monoclonal Antibody (HI30), Biotin, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

75.00 EUR - 196.00 EUR

Specifications

Antigen CD45
Clone HI30
Concentration 0.5 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Product Code Brand Quantity Price Quantity & Availability  
15269579
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Affymetrix eBioscience
13-0459-82
100 μg
196.00 EUR
100µg
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15259579
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Affymetrix eBioscience
13-0459-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HI30 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Specifications

Specifications

CD45
0.5 mg/mL
Monoclonal
Liquid
RUO
P08575
PTPRC
Primary
4° C, store in dark, DO NOT FREEZE!
PTPRC
HI30
Flow Cytometry
Biotin
Mouse
Human
5788
IgG1 κ
Affinity chromatography
Antibody
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