CD45.1, clone: A20, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0453-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Biotinylated A20 is recommended for IHC, cat. 13-0453).Applications Tested: The A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD45, L-CA, Lca, RT7, T200|
|Flow Cytometry, Immunoprecipitation|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.