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CD4, clone: GK1.5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0041-86

Additional Details : Weight : 0.01000kg

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Product Code. 15286747

  • 277.00 EUR / 1mg


Estimated Shipment date
from Supplier 27-09-2018
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Description

Description

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in isolation and depletion of CD4+ cells, blocking of ligand binding and T cell activation via CD4. (Please use Functional Grade purified GK1.5, cat. 16-0041, in functional assays.).Applications Tested: The GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P06332
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
GK1.5
Unconjugated
Cd4
Liquid
Cd4
IgG2b, kappa, kappa
1mg
4° C
Primary
12504
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