Learn More
CD33 Monoclonal Antibody (WM53), Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen MA516982
Additional Details : Weight : 0.01000kg
Description
Overnight incubation is recommended. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is lymphatic tissue. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Overnight incubation with primary antibody is recommended.
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.Specifications
CD33 | |
Monoclonal | |
1 mg/mL | |
PBS with 0.09% sodium azide | |
P20138 | |
CD33 | |
Human AML cells | |
100 μg | |
Primary | |
Human | |
Antibody | |
IgG1 |
Flow Cytometry, Immunoprecipitation, Western Blot | |
WM53 | |
Unconjugated | |
CD33 | |
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3 | |
Mouse | |
Protein G | |
RUO | |
945 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. | |
Liquid |