CD3, Alexa Fluor 700, clone: 17A2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 56-0032-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|Alexa Fluor 700|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|