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CD3, Alexa Fluor 700, clone: 17A2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0032-80

Additional Details : Weight : 0.25000kg

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Product Code. 15392090

  • 94.50 EUR / 25µg


In Stock 3
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Description

Description

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P04235, P11942, P22646, P24161
FLJ18683, T3E, TCRE
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
17A2
Alexa Fluor 700
Cd3g
Conjugated
Cd247, Cd3d, Cd3e, Cd3g
IgG2b, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12500, 12501, 12502, 12503
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