missing translation for 'onlineSavingsMsg'
Learn More

CD29 (Integrin beta 1), clone: KMI6, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0292-81

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15286887

  • 62.50 EUR / 50µg


Estimated Shipment date
from Supplier 29-05-2019
Add to basket

Description

Description

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The KMI6 antibody has also been tested by immunoblotting at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.
Specifications

Specifications

CD29 (Integrin beta 1)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P09055
FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, ITGB1, ITBG1D
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
KMI6
Unconjugated
Itgb1
Liquid
Itgb1
IgG2a, kappa
50 μg
4° C
Primary
16412
Videos

For Research Use Only.