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CD25 Monoclonal Antibody (eBio3C7 (3C7)), eFluor™ 450, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

94.75 EUR - 247.00 EUR

Specifications

Antigen CD25
Clone eBio3C7 (3C7)
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15301710
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Affymetrix eBioscience
48-0253-82
100 μg
247.00 EUR
100µg
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15391700
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Affymetrix eBioscience
48-0253-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody. Applications Reported: This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio3C7 (3C7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™.

CD25 Monoclonal antibody specifically detects CD25 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD25
0.2 mg/mL
Monoclonal
Liquid
RUO
P01590
IgG2b κ
Affinity chromatography
Antibody
eBio3C7 (3C7)
Flow Cytometry
eFluor 450
Rat
Mouse
16184
Primary
4° C, store in dark, DO NOT FREEZE!
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