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CD25 Monoclonal Antibody (eBio3C7 (3C7)), eFluor™ 450, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
94.75 EUR - 247.00 EUR
Specifications
Antigen | CD25 |
---|---|
Clone | eBio3C7 (3C7) |
Concentration | 0.2 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15301710
|
Affymetrix eBioscience
48-0253-82 |
100 μg |
247.00 EUR
100µg |
Estimated Shipment: 10-04-2024 Log in to see stock. |
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15391700
|
Affymetrix eBioscience
48-0253-80 |
25 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody. Applications Reported: This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio3C7 (3C7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™.
CD25 Monoclonal antibody specifically detects CD25 in Mouse samples. It is validated for Flow Cytometry.Specifications
CD25 | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P01590 | |
IgG2b κ | |
Affinity chromatography | |
Antibody |
eBio3C7 (3C7) | |
Flow Cytometry | |
eFluor 450 | |
Rat | |
Mouse | |
16184 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |
For Research Use Only.