CD215 (IL-15Ra), clone: eBioJM7A4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7159-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL-2Rbeta and gamma chains, which are essential for signal transduction. The IL-15Ralpha receptor is structurally related to IL-2Ralpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2Ralpha and IL-2. In the human, 3 differentially spliced human IL-15Ralpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL-15Ralpha, like that of IL-2Ralpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL-2Rbeta and IL-2Rgamma in the absence of the alpha subunit.Applications Reported: This eBioJM7A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).Applications Tested: This eBioJM7A4 antibody has been tested by flow cytometric analysis of LPS and IFN gamma-stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|IL-15RA, Interleukin-15 receptor subunit alpha, IL15RA, CD215|
|Flow Cytometry, Immunoprecipitation, Western Blot|
|Store at 2-8°C.|
For Research Use Only.