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BrdU, clone: BU20A, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5071-80

Additional Details : Weight : 0.01000kg

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Product Code. 15287397

  • 52.50 EUR / 25µg


Estimated Shipment date
from Supplier 28-05-2019
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Description

Description

Resuspend the cell pellet by pulse vortexing. Then add 1 mL of freshly prepared Foxp3 Fixation/Permeabilization solution (cat. 00-5521) to each sample. pulse vortex again.6. Incubate for 30 to 60 minutes at 2-8°C in the dark.7. Wash once by adding 2 mL 1X Permeabilization Buffer (made from 10X Permeabilization Buffer included in the Foxp3/Transcription Factor Staining Buffer Set) followed by centrifugation. Decant the supernatant.8. Resuspend the cell pellet with 100 µL Flow Cytometry Staining Buffer containing 30 µg of DNase I.9. Incubate for 1 hr at 37°C and then wash.10. Stain cells with anti-BrdU antibody for 30 min to 1 hr and then wash.10. If necessary, stain with secondary antibody for 30 min and then wash.12. Analyze the samples. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
Specifications

Specifications

BrdU
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
IgG1, kappa
25 μg
4° C
Primary
Chemical
Flow Cytometry, Immunohistochemistry (Frozen)
BU20A
Unconjugated
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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