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beta Catenin Monoclonal Antibody (15B8), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
79.00 EUR - 233.00 EUR
Specifications
Antigen | beta Catenin |
---|---|
Clone | 15B8 |
Concentration | 0.5 mg/mL |
Applications | Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15257327
|
Affymetrix eBioscience
14-2567-82 |
100 μg |
233.00 EUR
100µg |
Estimated Shipment: 29-05-2024 Log in to see stock. |
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15247327
|
Affymetrix eBioscience
14-2567-80 |
25 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells. Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 15B8 is recommended for use in intracellular flow cytometry.).
Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.Specifications
beta Catenin | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P35222, Q02248 | |
CTNNB1 | |
Primary | |
4° C | |
CTNNB1 |
15B8 | |
Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
Unconjugated | |
Mouse | |
Human, Mouse | |
12387, 1499 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.