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beta Catenin Mouse anti-Human, Mouse, Clone: 15B8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-2567-82

Additional Details : Weight : 0.01000kg

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Product Code. 15257327

  • 180.00 EUR / 100µg


Estimated Shipment date
from Supplier 26-07-2019
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Description

Description

Applications Tested: This 15B8 antibody has been tested by western blot analysis on reduced cell lysates prepared from the Jurkat cell line. This antibody can be used at 1-5 μg/mL. This antibody has been tested by immunohistchemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintece of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
Specifications

Specifications

beta Catenin
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q02248, P35222
Cadherin associated protein, Catenin beta 1, CATNB, CTNNB
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
15B8
Unconjugated
CTNNB1
Liquid
Ctnnb1
IgG1, kappa
100 μg
4° C
Primary
12387, 1499
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