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Invitrogen™ AMPK (A2/B2/G2) Recombinant Human Protein
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex.
Brand: Invitrogen™ PV6252
Additional Details : Weight : 0.01000kg
Description
AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKK β. Recombinant full-length human AMPK (combination of A2/B2/G2 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes available for convenient use
Specifications
| Enzyme and Protein Activity Assay, Industrial and Applied Science, Kinase Assay, Kinase Biology, Kinase Proteins and Expression Reagents, Pharma and Biopharma, Protein Assays and Analysis, Protein Biology, Target and Lead Identification and Validation | |
| 63.1/36/41kDa | |
| Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. | |
| Recombinant |
| 50mM sodium phosphate pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol | |
| 1mg | |
| Human | |
| His Tag |