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Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Brand: Invitrogen™ 11753100
475.20 EUR valid until 2023-12-15
Use promo code "21275" to get your promotional price.
Additional Details : Weight : 0.05000kg
Description
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
• Efficiency: offers significant time and cost savings for increased throughput
• Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
• Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells
• Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Streamline your workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for your qRT-PCR application
CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).
This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.
Specifications
| • 10 mL Resuspension Buffer • 1 mL Lysis Enhancer • 500 μL DNase I, Amplification Grade (1 U/μL) • 160 μL 10X DNase I Buffer • 400 μL 25 mM EDTA • 100 μL SuperScript™ III RT/Platinum™ Taq Mix • 2 × 1.25 mL 2X Reaction Mix • 1 mL 50 mM MgSO4 • 2 mL DEPC-treated water • 10 μL HeLa Total RNA (10 ng/μL) • 100 μL ROX™ Reference Dye (store at -20°C in the dark) The kit is shipped on dry ice and should be stored at -20°C |
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| Fast, Standard | |
| CellsDirect™, Platinum™, SuperScript™ | |
| 2X | |
| Cells |
| Primer-probe | |
| 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system | |
| RT-PCR | |
| 100 reactions |
For Research Use Only. Not for use in diagnostic procedures.