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Invitrogen™ ACVR2A Human Protein

Easily screen for kinase binding. Thermo Fisher Invitrogen™ ACVR2A Human Protein recombinant fusion protein consists of full-length activin A receptor kinase and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.

Brand:  Invitrogen™ PR9111A

Additional Details : Weight : 0.01000kg

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Product Code. 15458713

  • 1286.00 EUR / 100µg


Estimated Shipment date
from Supplier 01-10-2018
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Description

Description

The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.

  • Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
  • Expressed in insect cells
  • GST tagged for use with Eu-labeled GST antibody
  • No special measures were taken to activate this kinase

ACVR2A for the LanthaScreen Kinase Binding Assay
Activin A receptor kinase (ACVR2A) is a type II member of the TGF-beta family of receptor Serine/Threonine kinases. ACVR2A is involved in the activin and myostatin signaling pathway. (ACVR2A Aliases: ACVR2, ACTRII).

This insect cell-expressed recombinant fusion protein of full-length ACVR2A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST reagents.

Specifications

Specifications

NP001607.1
Frozen
85.3kDa
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
Full Length
Kinase Assay
STKR Kinase Family
Yes
Protein Biology|Enzyme & Protein Activity Assays|Kinase Activity|Protein Assays and Analysis
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol.
100μg
RUO - research use only
ACVR2A, ACTR2, ACTRII, ACVR2
Baculovirus
Human
GST Tag
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