Electrophoresis, Western Blotting and ELISA

GE Healthcare PhastGel™ Precast Gels

Select from homogeneous and gradient polyacrylamide gels. GE Healthcare PhastGel™ Precast Gels save time and improve reproducibility. X10 PHASTGEL HIGH DENSITY

Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Prestained Protein

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE). X2 PageRuler(TM) Unstained Protein (2 x 250 uL)

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis. SUPERSIGNAL WEST FEMTO CHEMILUMINESCENT SUBSTRATETrial kit, SuperSignal 20mL Store in Fridge at 4C

Thermo Scientific™ HisProbe™-HRP Reagent and Kit

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative. 2 MG INDIA HISPROBETM-HRP

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Molecular Probes™ CellROX™ Deep Red Reagent

Optimized probe for detection of oxidative stress in cells X5 CellROX(TM) Deep Red reagent, for oxidativeEA=5x50µL

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Plus Prestained Protei

Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis. X2 Fermentas PageRuler(TM) Low Range Unstained

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. ECL PRIME WESTERN BLOTTING DET1000 cm2 membrane

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 17-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 17-well

MetaPhor™ Agarose

500GR MetaPhor Agarose

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 5 LT RESTORE(TM) WESTERN BLOT STRIPPING BUFFER 5L

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 6.7 TO 7.725mL Store in Fridge at 4C

Lonza™ SeaPlaque™ Agarose

  • For preparative DNA and RNA electrophoresis
  • Molecular biology grade
  • ≤0.10 EEO
  • Low melting temperature argarose (26°–30°C Gelling temperature)
  • DNase/RNase-free
125GR SeaPlaque Agarose

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 15-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 15-well

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. 50 ML ECL WESTERN BLOTTING SUBSTRATE

Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X2 Fermentas PageRuler(TM) Broad Range Unstained

GE Healthcare pI Markers

Run these markers in parallel with the experimental samples on isoelectric focusing gels. GE Healthcare Life Sciences™ pI Markers are lyophilized mixtures of stable, salt-free, highly purified proteins. IEF MARKER, HIGH PI CALIBRATION KIT PH 5 TO 10.50 Store in Fridge at 4C

GE Healthcare Amersham™ Hybond™ -XL Membranes

Use for optimal signal-to-noise ratios when using radioactively labeled probes. GE Healthcare Life Sciences™ Amersham™ Hybond™ -XL Membranes are novel, charged nylon membranes that have a greater concentration and a more even distribution of positive charge than other charged nylon membranes. HYBOND-XL,10X22CMX22CM 10 SHEETS

Hoefer™ SG Series Gradient Makers

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride GRADIENTKÄRL 50ML

eBioscience™ Human/Mouse TGF beta 1 ELISA Ready-SET-Go!™ Kit, 2nd Generation

Specifically engineered for accurate and precise measurement of human or mouse TGF beta 1 protein levels from samples including serum, plasma, and cell culture supernatants. This second generation kit has increased sensitivity with a range of 8-1000pg/mL. X10 Human/Mouse TGF beta 1 ELISA Ready-SET-Go! (2nd Generation) (supplied as 10 x 96 tests)

Lonza™ NuSieve™ GTG Agarose

NuSieve GTG FP=125 g

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis 200ML SuperSignal West Pico PLUS ChemiluminescentSubstrate

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ Molecular Probes™ BODIPY™ 581/591 C11

Lipid peroxidation sensor 4,4-DIFLUORO-5-(4-PHENYL-BODIPY® 581/591 undecanoic acid can be used to

Thermo Scientific™ Pierce™ Silver Stain Kit for Mass Spectrometry

Reversibly stain proteins in polyacrylamide gels to identify bands to excise; then destain the gel pieces for analysis by mass spectrometry. SILVERSNAP STAIN MASS SPECTROMETRY

EMD Millipore™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls. X10 Immobilon-P 26 x 26cm sheet PVDF .45um

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used. ECL BLOTTING REAGENTS

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background. X1 Amersham Protran 0.1um Nitrocellulose 200mm x 4m roll

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