Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE). X2 PageRuler(TM) Unstained Protein (2 x 250 uL)

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Plus Prestained Protei

GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. X25 Amersham Hybond 0.45um PVDF 80mm x 90mm sheets

Thermo Scientific™ Pierce™ PageBlue™ Protein Staining Solution

A sensitive formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid. PAGEBLUE PROTEIN STAINING SOLUTIONin Fridge at 4C

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Prestained Protein

Invitrogen™ Novex™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs 500 ML TMB SINGLE SOLUTION (ELISA) ZYMED(TM)Invitrogen SKU 002023 500mL

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. 50 ML ECL WESTERN BLOTTING SUBSTRATE

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. ECL PRIME WESTERN BLOTTING DET1000 cm2 membrane

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis. SUPERSIGNAL WEST FEMTO CHEMILUMINESCENT SUBSTRATETrial kit, SuperSignal 20mL Store in Fridge at 4C

Invitrogen™ Molecular Probes™ CellROX™ Deep Red Reagent

Optimized probe for detection of oxidative stress in cells X5 CellROX(TM) Deep Red reagent, for oxidativeEA=5x50µL

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 5 LT RESTORE(TM) WESTERN BLOT STRIPPING BUFFER 5L

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background. X1 Amersham Protran 0.1um Nitrocellulose 300mm x 4m roll

Hoefer™ SG Series Gradient Makers

Ideal for generating gradients of polyacrylamide, sucrose, and cesium chloride GRADIENTKÄRL 50ML

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 4.2 TO 4.925mL Store in Fridge at 4C

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 15-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 15-well

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 10-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 10-well

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis 200ML SuperSignal West Pico PLUS ChemiluminescentSubstrate

Invitrogen™ Novex™ XCell SureLock™ Replacement Parts

For the XCell SureLock Mini Blot Module L-BUFFER CHAMBER SURELOCKReplacement Parts for Electrophoresis Apparatus

Agarose (Low-Melting, <1kb DNA/RNA/Genetic Analysis Grade), Fisher BioReagents

100GR Agarose (Low-Melting, less than 1kb DNA/RNA/Genetic Analysis Grade)

GE Healthcare Amersham™ Hybond™ -XL Membranes

Use for optimal signal-to-noise ratios when using radioactively labeled probes. GE Healthcare Life Sciences™ Amersham™ Hybond™ -XL Membranes are novel, charged nylon membranes that have a greater concentration and a more even distribution of positive charge than other charged nylon membranes. HYBOND-XL MEMBRANE, 30CM X 3MROLL

Invitrogen™ Luminex™ Cytokine Human Magnetic 30-Plex Panel

Specifically designed for quantifying human cytokines, chemokines and growth factors in serum, plasma, and tissue culture supernatant HU MAGNETIC 30-PLEX KIT

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. SUPERBLOCK BLOCKING BUFF IN TBS 1LTemperature Store in Fridge at 4C

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Normal Goat Serum Control, Invitrogen™

Normal Goat Serum Control, 10000C, from Invitrogen™. Species Reactivity: Goat; Applications: Control. 25 ML NORMAL GOAT SERUM. CLASS: RUOSTATEMENT: FORresearch useonly. Not for use in diagnostic proced

Invitrogen™ Mini Gel Tank and Blot Module Set

Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels Mini Gel Tank and Blot Module Set

Agarose (Low-Melting, Nucleic Acid Recovery/Molecular Biology Grade), Fisher BioReagents

25GR Agarose, low melting, nucleic acid recovery,Molecular biology grade

Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X2 Fermentas PageRuler(TM) Broad Range Unstained

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used. ECL BLOTTING REAGENTS

Thermo Scientific™ Pierce™ Mouse IFN gamma ELISA Kits

Measure levels of mouse interferon gamma (IFNg) in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates. MOUSE IFNG ELISA

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