Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Thermo Scientific™ Pierce™ PageBlue™ Protein Staining Solution

A sensitive formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid. PAGEBLUE PROTEIN STAINING SOLUTIONin Fridge at 4C

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 15-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 15-well

Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results. Pierce Silver Stain Kit

GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. X25 Amersham Hybond 0.45um PVDF 100mm x 100mm sheets

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. 50 ML ECL WESTERN BLOTTING SUBSTRATE

Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Prestained Protein

Fisherbrand™ Power Supply, Mini 300V Plus

Provides up to 300V (volts) and 400mA of power. Fisherbrand™ Power Supply, 300V Mini Plus includes dual voltage compatibility. POWER SUPPLY MINI300V PLUS 300V, 400mA, 60W,twin output, constant current/voltage

Invitrogen™ Novex™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs 500 ML TMB SINGLE SOLUTION (ELISA) ZYMED(TM)Invitrogen SKU 002023 500mL

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ Novex™ Mark12™ Standard, Unstained

Allows the closest estimation of molecular weight of your sample protein over a wide molecular weight range 1 ML UNSTAINED PROTEIN STANDARD MARK12(TM) WIDE-RAnge for SDS-PAGE 1mL Store at 2-8C

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. X10 PageRuler(TM) Plus Prestained Protei

GE Healthcare PhastGel™ Precast Gels

Select from homogeneous and gradient polyacrylamide gels. GE Healthcare PhastGel™ Precast Gels save time and improve reproducibility. X10 PHASTGEL HIGH DENSITY

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE). X2 PageRuler(TM) Unstained Protein (2 x 250 uL)

Invitrogen™ Mini Gel Tank and Blot Module Set

Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels Mini Gel Tank and Blot Module Set

GE Healthcare Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls

Use for robust protein blotting. GE Healthcare Life Sciences™ Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background. X1 Amersham Protran Supported 0.2um Nitrocellulose 200mm x 4m roll

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 17-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 17-well

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis. SUPERSIGNAL WEST FEMTO CHEMILUMINESCENT SUBSTRATETrial kit, SuperSignal 20mL Store in Fridge at 4C

Invitrogen™ Novex™ XCell SureLock™ Replacement Parts

For the XCell SureLock Mini Blot Module PLATINUM METAL WIRE 305MM LENGTH 0.4µM DIAMETER 00

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder

A mixture of eight blue-, green- and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting. X2 Fermentas Spectra(TM) Multicolor High Range

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 6.7 TO 7.725mL Store in Fridge at 4C

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. X3 AMERSHAM ECL PRIME, CHEMILUMINESCENT WESTERNblotting detection reagent 3mLStore in Fridge at

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used. ECL WESTERN BLOT DETECTION REAGENT

Whatman™ Gel Blotting Paper Grade GB003

A thick paper ideal for lysis/denaturation of colony or plaque lifts and Western blots X100 GB003 100X150MM 100/PK, 100/CS, MA: 10427805

UVP Ultra Violet Product™ Gel Imaging System

Use this system for imaging fluorescent gels, visible stains, autoradiograph film and colony plates. UVP™ Gel Imaging Systems include a high resolution scientific grade camera with real-time preview and the capability to image western blots. Gel imaging system GelDoc-It(R) 315 zoom lensEth Br filter, USB card /cable2 mpixel CCD camera,

Agarose (Low-Melting, <1kb DNA/RNA/Genetic Analysis Grade), Fisher BioReagents

100GR Agarose (Low-Melting, less than 1kb DNA/RNA/Genetic Analysis Grade)

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 10-Well

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions X10 Bolt 4-12 percent Bis-Tris Plus Gels, 10-well

Thermo Scientific™ Pierce™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB) 100 ML TMB SUBSTRATE SOLUTION 100ML STORE INfridge at 4C

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