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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Corning™ PYREX™ Cloning Cylinders
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Create identical cells by surrounding a single cell or group of cells
Product Type | Cloning Cylinder |
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Material | Borosilicate Glass |
Sterility | Non-sterile |
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Bel-Art™ Cloning Cylinders
Manufactured from polystyrene. SP Scienceware™ Cloning Cylinders facilitates the isolation of individual cell clones.
Product Type | Cloning Cylinder |
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Material | Polystyrene |
Sterility | Sterile |
Invitrogen™ Zero Blunt™ PCR Cloning Kit
Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases
Promoter | T7 |
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Content And Storage | Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt PCR |
Bacterial or Yeast Strain | TOP10 |
Vector | Blunt DNA Cloning Vectors |
Product Line | Zero Blunt™ |
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Kanamycin (KanR), Zeocin™ (ZeoR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPO™-TA |
Product Line | TOPO™ |
Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells
Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases
Promoter | T7 |
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Content And Storage | Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt PCR |
Bacterial or Yeast Strain | Not Included |
Vector | Blunt DNA Cloning Vectors |
Product Line | TOPO™, Zero Blunt™ |
Promoter | T7, SP6 |
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Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Cloning Method | TOPO™-TA |
Vector | TOPO-TA Vectors |
Product Line | TOPO™ |
Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Content And Storage | Dry Ice (-80°C) |
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Description | Bacterial/Yeast Strain: DH10B |
For Use With (Application) | Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS) |
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing
Shipping Condition | Dry Ice |
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Promoter | T7, T3 |
Content And Storage | Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
Vector | TOPO Cloning Vectors |
Product Line | TOPO™ |
Invitrogen™ pLenti6/V5 Directional TOPO™ Cloning Kit
Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Promoter | T7 |
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Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | Not Included |
For Use With (Application) | PCR Cloning |
Vector | pCR2.1 |
Product Line | TA Cloning™ |
Thermo Scientific™ pENTR™/D-TOPO™ Cloning Kit
Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System
Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit
Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.