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Cytiva Sepharose™ Fast Flow Cation Exchange Media
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
| Particle Size | 45 to 165 μm |
|---|---|
| pH Range | 4 to 13 |
Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Flow Rate | 1 to 4 mL/min |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | HiTrap Talon crude prepacked Column |
| Material | Polypropylene |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
| Max. Pressure | 3 bar |
Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin
Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | Strep-Tactin XT |
| Density (Metric) | Approx. 5 mg/mL resin |
| Matrix | Rigid, cross-linked agarose, spherical |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 6 to 10 pH |
| Binding Capacity | Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin |
| Storage Requirements | 2 to 8°C in 20% ethanol |
| For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag |
Cytiva CNBr-Activated Sepharose™ 4 Fast Flow Affinity Media
Used for coupling of large amino-containing ligands
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media
Used for purifying proteins and peptides that have an affinity for metal ions. Cytiva IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
| Product Type | IMAC Sepharose 6 Fast Flow Media |
|---|---|
| For Use With (Application) | For Purifying Proteins and Peptides That Have an Affinity for Metal Ions |
Cytiva HiTrap™ Capto™ PlasmidSelect
For the purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications
| Matrix | Capto™ PlasmidSelect |
|---|---|
| Length (Metric) | 2.5 cm |
| Product Type | Chromatography Resin |
| For Use With (Application) | Purification of Supercoiled DNA From Research Scale to cGMP Production |
| Max. Pressure | 5 bar (0.5 MPa) |
Cytiva Q Sepharose™ XL
Hydrophilic base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in elution pool
Cytiva TALON™ Superflow Medium
Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | Talon Superflow Media |
| Binding Capacity | Up to 20 mg Histidine-tagged Protein |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography |
Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media
Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases
Cytiva HiScreen Capto Butyl ImpRes
HiScreen Capto Butyl ImpRes is a ready-to-use column prepacked with Capto Butyl ImpRes hydrophobic interaction chromatography resin and is part of Cytiva’s process development platform.
Cytiva Capto™ adhere ImpRes Multimodal BioProcess™ Chromatography Medium
For high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules
| Includes | Certificate of Analysis |
|---|---|
| Particle Size | 40 μm |
| Ligand Type | Multimodal Strong AnIon Exchanger |
| Matrix | High-flow Agarose |
| Stability | 1 M Acetic Acid, 1 M NaOH, 2 M NaCl, 5% 1-Propanol, 30% Isopropanol, 70% Ethanol |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | Capto Adhere ImpRes Media |
| Binding Capacity | 45 to 85 mg Mab/mL |
| Anion Exchange Capacity | 0.08 to 0.11 mM Cl-/mL |
| Storage Requirements | 4°C to 30°C |
| For Use With (Application) | For High Resolution Polishing of Monoclonal Antibodies (MAbs) and Other Biomolecules |
| Max. Pressure | 300 kPa |
Cytiva Capto™ MMC ImpRes Multimodal BioProcess™ Chromatography Medium
For high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules
| Particle Size | 40 μm |
|---|---|
| Ligand Type | Multimodal Weak CatIon Exchanger |
| Matrix | High-flow Agarose |
| Stability | 1 M Acetic Acid, 1 M NaOH, 2 M NaCl, 5% 1-Propanol, 30% Isopropanol, 70% Ethanol |
| Storage Buffer | 20% Ethanol, 0.2 M Sodium Acetate |
| pH Range | 3 to 12 |
| Product Type | Capto MMC ImpRes Media |
| Binding Capacity | 60 to 90 mg Mab/mL |
| Anion Exchange Capacity | 25 to 39 μM/mL |
| Storage Requirements | 4°C to 30°C |
| For Use With (Application) | For High Resolution Polishing of Monoclonal Antibodies (MAbs) and Other Biomolecules |
| Max. Pressure | 300 kPa |
Cytiva Capto™ Multimodal (MMC) Chromatography Media
Used to solve specific purification challenges at both high and low ionic strengths
| Particle Size | 75 μm |
|---|---|
| Product Type | Capto MMC Media |
Cytiva Butyl Sepharose™ 4 Fast Flow Chromatography Media
Used for capture and intermediate purification of larger proteins
| Matrix | Based on cross-linked 4% agarose |
|---|---|
| Product Type | Butyl Sepharose 4 Fast Flow Media |