Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gibco™ HBSS, calcium, magnesium
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Gibco™ HBSS (10X), no calcium, no magnesium, no phenol red
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Gibco™ DPBS, calcium, magnesium
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Gibco™ PB-MAX™ Karyotyping Medium
Fully supplemented medium developed for short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures
Gibco™ EBSS, no calcium, no magnesium, no phenol red
Earle's Balanced Salt Solution (EBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Gibco™ DPBS, calcium, magnesium, glucose, pyruvate
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Gibco™ HBSS, no calcium, no magnesium
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Gibco™ DPBS (10X), calcium, magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Gibco™ PBS, pH 7.4
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
Gibco™ PBS, pH 7.4
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.