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Products

IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension

Ideal for gravity flow purification of recombinant Strep-tag™ proteins. IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension is conveniently formulated as a 50% Sepharose™ suspension. 20 ML Strep-Tactin(R) Sepharose 50 % suspension

IBA Solutions For Life Science 5-Methyl-CTP

A modified nucleoside-triphosphate (modified 2´-ribonucleoside-5´-triphosphates) that helps in studying RNA-protein interactions. 5-Methyl-CTP

IBA Solutions For Life Science 2'-dCTP alpha S

A modified nucleoside-triphosphate (alpha-Thio-nucleoside-5´-triphosphate) that allows researchers to study site-directed mutagenesis, stabilizing DNA against nucleases, X-ray or NMR analysis of enzyme-substrate complexes. 2'-dCTP alpha S

IBA GmbH™ CMV pp65 peptide NLVPMVATV (HLA-A*0201)

IBA GmbH™ CMV pp65 peptide NLVPMVATV (HLA-A*0201) is a single peptide for stimulation of human CMV-specific CD8+ T-cells. 1MG CMV pp65 peptide NLVPMVATV (HLA-A*0201)

IBA GmbH™ Strep-Tactin™ APC for MHC I Streptamers™

IBA GmbH™ Strep-Tactin™ APC for MHC I Streptamers™ is for staining/FACS isolation of antigen-specific cytotoxic T cells (CD8). Strep-Tactin APC for MHC I Streptamers; 50 µl

IBA GmbH™ Strep-Tactin™XT Spin Column Kit

IBA GmbH™ Strep-Tactin™XT Spin Column Kit allows for rapid parallel purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins. 1 KIT Strep-Tactin XT Spin Column Kit

IBA Solutions For Life Science C8-Alkyne-dUTP

A modified nucleoside-triphosphate (3'-modified oligos) that is suitable for subsequent modifications with fluorescent or non-fluorescent Azides. C8-Alkyne-dUTP

IBA Solutions For Life Science™ Avidin

15 MG Avidin 15mg Store in -20C Freezer

IBA Solutions For Life Science™ Streptavidin (not for Strep-tag applications) store in -20'C freezer IBA

100 MG Streptavidin (not for Strep-tag applications) 100mg Store in -20C Freezer

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension can be used to pack your own columns for protein purification. Strep-Tactin XT Sepharose 50% suspension

IBA Solutions for Life Science™ Strep-tag™ Washing Buffer, 10X

100 ML Strep-tag(R) washing buffer (10x buffer W)

IBA Solutions for Life Science™ Strep-tag™ Protein Purification Buffer Set

100ml Strep-Tactin purification buffer set(100mL 10x buffer W, 25mL 10x buffer E, 100mL

IBA Lifesciences™ Biotin Blocking Buffer

2 ML BIOTIN BLOCKING BUFFER 2ML STORE IN 4C FRIDGE

IBA Solutions for Life Science™ Anhydrotetracycline

CAS: 1665-56-1 Molecular Formula: C22H22N2O7 Molecular Weight (g/mol): 426.43 InChI Key: JGRMIMMCZBOWCW-LCGGBVFXSA-N Synonym: anhydrotetracycline, 5a,6-anhydrotetracycline, 4s-anhydrotetracycline, cxcveerymjzmmm-docrcchosa-n, 4s,4as,12as-4-dimethylamino-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4a,5,12,12a-hexahydrotetracene-2-carboxamide, anhydrotetracycline hydrochloride, 2-naphthacenecarboxamide, 1,4,4a,5,12,12a-hexahydro-4-dimethylamino-1,12-dioxo-6-methyl-3,10,11,12a-tetrahydroxy-, 4s-4-alpha,4a-alpha,12a-alpha, 2-naphthacenecarboxamide,4-dimethylamino-1,4,4a,5,12,12a-hexahydro-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-, 4s,4as,12as, anhydrotetracyclin, tdc PubChem CID: 54675758 50 MG ANHYDROTETRACYCLINE 50MG STORE IN -20CFreezer

IBA Solutions for Life Science™ D-Biotin

2 GR D-Biotin (not for Strep-tag applications) 2g

IBA GmbH™ Small Mammalian Expression Vector

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA GmbH™ Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system. pDSG-IBA105; 5µg

IBA Solutions For Life Science™ D-Desthiobiotin, 0.107g for 200mL 1x buffer store in 4'C refrigerator IBA

0.107 GR D-DESTHIOBIOTIN, 0.107G FOR 200ML 1Xbuffer 00 Store in 4C Fridge

IBA GmbH™ BioLock Biotin Blocking Solution

IBA GmbH™ BioLock Biotin Blocking Solution is a ready to use and cost effective solution for the removal of free biotim from cell culture supernatant. BioLock - Biotin blocking solution; 250 ml

IBA GmbH™ E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA GmbH™ E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli. pASG-IBA45; 5µg

IBA Solutions For Life Science™ CpG-dinucleotide IBA

1 MMOL CpG-dinucleotide 1µmol Store in Freezer at

IBA GmbH™ CMV pp65 peptide TPRVTGGGAM (HLA-B*0702)

IBA GmbH™ CMV pp65 peptide TPRVTGGGAM (HLA-B*0702) is a single peptide for stimulation of human CMV pp65-specific CD8+ T-cells. 1MG CMV pp65 peptide TPRVTGGGAM (HLA-B*0702)

IBA Solutions For Life Science 2'-dATP alpha S

A modified nucleoside-triphosphate (alpha-Thio-nucleoside-5´-triphosphate) that allows researchers to study site-directed mutagenesis, stabilizing DNA against nucleases, X-ray or NMR analysis of enzyme-substrate complexes. 2'-dATP alpha S

IBA Solutions For Life Science™ UpU-dinucleotide IBA

5 MG UpU-dinucleotide 5mg Store in Freezer at -20C

IBA Solutions For Life Science StarGate™ pLSG-IBA Acceptor Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination. pLSG-IBA25; 5µg

IBA Solutions For Life Science StarGate™ pYSG-IBA Acceptor Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter. pYSG-IBA45; 5µg

IBA Solutions For Life Science Bio dUTP

A modified oligonucleotide (3’-modified oligos) that is used in cloning and expression applications. Bio dUTP

IBA Solutions For Life Science PH-CpU-Cyanine 5 Transcription Starter

An RNA dinucleotide (oligonucleotide for 5´-labeled transcripts) that is very useful for transcription analysis. PH-CpU-Cyanine 5

IBA GmbH™ WET FRED for Gravity Flow Columns

IBA GmbH™ WET FRED for Gravity Flow Columns is an aid to facilitate application of large cell culture volumes to gravity flow columns. WET FRED 5-10ml Adapter for 5-10ml Gravity flow columns

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Featured Products

Twin-Strep-tag™/Strep-Tactin™XT


Twin-Strep-tag

The purification system with the highest affinity – one tag for expression, purification, detection and immobilization

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Immobilization with near covalent binding affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR experiments »

  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated

High affinity meets high capacity!


High affinity meets high capacity!

Strep-Tactin™XT Superflow™ high capacity »

  • Higher protein yields compared to all other Strep-Tactin™ resins
  • Dynamic binding capacity of ~15 mg protein per ml resin
  • Elution of concentrated protein due to small elution volumes
  • Cost efficent: small price for more protein

Try our new high affinity Strep-tag™ system


Try our new high affinity Strep-tag® system

Starter Kit consists of:

  • Strep-Tactin™XT Superflow™ gravity flow column
  • Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
  • Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
  • Strep-Tactin™ conjugated with HRP (for Western blot detection)
  • Short protocol
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free cells
  • Directly from whole blood
  • No centrifugation

Fab-TACS™ Gravity and FABian™ »

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™ »

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™ »

  • Completely reversible reagents
  • Detachment from the cells at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Non-magnetic and bead-free

Peptides for T cell stimulation »

  • Purity > 80 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ »

Detection of receptor-specific T cells with Fab Streptamers™ »

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning & Transfection


Cloning & Transfection

More than 150 expression vectors »

  • Variety of affinity tags
  • Expression hosts:  E. coli, mammalia, insect cells, yeast
  • StarGate cloning system

Mammalian Expression


The MEXi system »

Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.

Protein Purification


Protein Purification

Strep-tag™ system from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA »

  • Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
  • Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated

Immunoprecipitation


Selector resins based on high-affinity single-domain antibodies (sdAb)

  • High capacity
  • Minimal unspecific binding
Oligos
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DNA


RNA


DNA & RNA

Standard DNA oligonucleotides

  • Long range up to 200 bases*
  • Large scale oligonucleotides
  • 3’-, 5’- or internally modified

Random RNA, dsRNA, siRNA, miRNA

  • Long range up to 100 bases*
  • 3’-, 5’- or internally modified
  • 2‘-modified: -O-Me, -F, -NH2
  • Nucleoside Triphosphates
  • RNA and RNA/DNA hybrid
  • Dinucleotides

*depending on sequence and scale

Fluorescence Labels


Non-Fluorescence Labels


Fluorescence Labels

E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami

  • For dual and multiply labeled DNA and RNA
  • Excellent high quality 
  • More than 200 labels to choose from
  • Quenchers such as BBQ and BHQ™
  • Completely new dye combinations available

For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more

  • More than 80 internal, 5’- and 3’- DNA and RNA modifications 

Backbone Modifications


Aptamers


Backbone Modifications
  • Phosphorothioates

Custom synthesis of DNA and RNA aptamers

  • High affinity 
  • Low immunogenicity
Resources Strep-Tactin®XT Sample Request
IBA_about_us

About IBA GmbH

Solutions For Life Sciences

IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.