missing translation for 'onlineSavingsMsg'
Learn More
Learn More
LanthaScreen™ IkB alpha GripTite™ Cell Line
LanthaScreen™ IkB alpha GripTite™ Cell Line contains a fusion protein consisting of the cDNA encoding for GFP and IkB under the control of a CMV promoter.
Brand: LanthaScreen™ K1681B
Additional Details : Weight : 0.50000kg
Description
- The LanthaScreen™IkB alpha GripTite™ cell line contains a fusion protein consisting of the cDNA encoding for GFP and IkB (GFP is fused to the N-terminus of IkB alpha) under the control of a CMV promoter stably transfected into GripTite™ cells.
- GripTite™ is a modified HEK293 cell line, which is a human kidney cell line which shows inducible activation of the NFkB signaling pathway by a number of different ligands, such as TNF and IL-1.
- Activation of the NFkB pathway involves the activation of IKK resulting in the phosphorylation of its target protein IkB alpha.
- This LanthaScreen cell line allows therefore the analysis of IKK activity using GFP-IkB alpha phosphorylation as readout.
- The GFP-IkB alpha construct was transfected into GripTite™ cells using Lipofectamine™2000, followed by selection with Blasticidin.
- This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker.
- This cell line is validated for EC50 and Z' under optimized conditions using TNF as ligand for IKK mediated GFP-IkB alpha phosphorylation or ubiquitination.
- This cell line has also been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time.
- Additional information using alternate stimuli is also available.
Specifications
| Store in liquid nitrogen immediately upon receipt, or thaw for immediate use | |
| Kinases, Signaling Pathway | |
| 1 Vial | |
| Dividing Cells |