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Proteintech™ Human PD1 ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous PD1
Brand: Proteintech™ KE00075-96T
This item is not returnable.
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Description
- Recognizes human PD1
- PD1 (Programmed cell death 1, CD279) is an immunoinhibitory receptor that belongs to CD28/CTLA-4 subfamily of Ig superfamily
- Is a 288 amino acid Type I transmembrane protein composed of one Ig superfamily domain, stalk, transmembrane domain and intracellular domain containing an ITIM and ITSM
- Expressed on T cells, B cells, natural killer T cells, activated monocytes, dendritic cells
- Engagement by ligands transduces a signal that inhibits T cell proliferation, cytokine production and cytolytic function
- Critical for regulation of T cell function during tolerance, autoimmunity and infection
- Blockade of PD1 can overcome immune resistance and shown to have antitumor activity
Process:
- Antibody specific for PD1 pre-coated onto microwells
- PD1 protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody specific for PD1 is added to detect captured PD1 protein
- For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
NP_005009 | |
43 pg/mL | |
Sandwich ELISA, Double Antibody | |
Plasma, Serum | |
Human | |
Human | |
<10% | |
Blue ice | |
3.5 hr. |
125 to 8000 pg/mL | |
ISO 13485 | |
Coated ELISA Kit | |
DAB | |
5133 | |
Solid | |
<10% | |
-20°C to +4°C | |
Q15116 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Human PD1 ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine